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  • Kuhner Shaker, Inc. - ISIS-Software
    holder Clamps Test tube holders High capacity tube holders Sticky mats Floor stands ISIS Software Interfaces Shelves Water baths Details Published on Monday 19 August 2013 15 08 ISIS Software For convenient supervision of the shaker Description Download Description Kuhner developed the user friendly ISIS Software for supervision monitoring and recording of data The software is easy to operate Further information can be found under kuhner technology Top Test version

    Original URL path: http://www.kuhnershaker.us/products/accessory/software/isis-software (2016-04-27)
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  • Kuhner Shaker, Inc. - Interfaces
    Microtiter Trays Microtiter Tray Trays with rubber mat Trays with sticky mats Trays with support bars Separating vessels trays Centrifuge tube trays Microtiter plate holder Clamps Test tube holders High capacity tube holders Sticky mats Floor stands ISIS Software Interfaces Shelves Water baths Details Published on Monday 19 August 2013 15 09 Interfaces Kuhner shakers are equipped with a CAN bus Description Description Kuhner shakers are equipped with a CAN

    Original URL path: http://www.kuhnershaker.us/products/accessory/software/interfaces (2016-04-27)
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  • Kuhner Shaker, Inc. - More
    tube holders High capacity tube holders Sticky mats Floor stands ISIS Software Interfaces Shelves Water baths Details Published on Monday 19 August 2013 15 09 Shelves Kuhner incubator shakers and the rack system can be fitted with shelves Description Ordering table Description The Kuhner LT X ISF1 X and ISF4 X incubator shakers can be fitted with a shelf allowing cultivation in petri dishes One or two shelves can be

    Original URL path: http://www.kuhnershaker.us/products/accessory/more (2016-04-27)
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  • Kuhner Shaker, Inc. - Shelves
    tube holders High capacity tube holders Sticky mats Floor stands ISIS Software Interfaces Shelves Water baths Details Published on Monday 19 August 2013 15 09 Shelves Kuhner incubator shakers and the rack system can be fitted with shelves Description Ordering table Description The Kuhner LT X ISF1 X and ISF4 X incubator shakers can be fitted with a shelf allowing cultivation in petri dishes One or two shelves can be

    Original URL path: http://www.kuhnershaker.us/products/accessory/more/shelves (2016-04-27)
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  • Kuhner Shaker, Inc. - Water bath
    with support bars Separating vessels trays Centrifuge tube trays Microtiter plate holder Clamps Test tube holders High capacity tube holders Sticky mats Floor stands ISIS Software Interfaces Shelves Water baths Details Published on Monday 19 August 2013 15 09 Water baths Stainless steel bath to reduce evaporation in shaken containers Description Ordering table Description To reduce evaporation from shake flasks or microtiter plates a stainless steel water bath can be

    Original URL path: http://www.kuhnershaker.us/products/accessory/more/water-bath (2016-04-27)
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  • Kuhner Shaker, Inc. - Online measurement
    v v pH 2 10 Cross sensitivity typically no cross sensitivity in culture media reduced to ionic strength salinity a high concentration of small florescent molecules in the visible range can interfere Storage stability 18 months provided the sensor is stored in the dark 18 months provided the sensor is stored in the dark Calibration sensor flask are pre calibrated one point calibration is possible at the beginning of the experiment when the medium conditions O 2 are defined sensor flask are pre calibrated one point calibration is possible at the beginning of the experiment when the medium conditions pH are defined Sterilization sensor flask are delivered irradiated sensor flask are delivered irradiated Resterilization steam sterilization ethylene oxide gamma irradiation is not recommended Top Application fields Application fields Online measurement of dissolved oxygen and pH S cerevisiae E coli CHO cell culture Saccharomyces cerevisiae Crabtree effect and consumption of ethanol Due to its overflow metabolism Crabtree effect baker s yeast Saccharomyces cerevisiae has the ability to produce ethanol from glucose even in the presence of oxygen After total consumption of the sugar accumulated ethanol can be oxidized as an energy source by baker s yeast under aerobic conditions With the help of the BPM system the impact of baffles on the oxygen supply to yeast cells was investigated The above mentioned diauxic growth of S cerevisiae with glucose and ethanol is illustrated with data generated by the BPM Fig 1 Dissolved oxygen concentration DO and pH value during cultivation of Saccharomyces cerevisiae in shake flasks Corning 250ml with and without baffles YEP medium with 20g L glucose 200rpm shaking frequency 50 mm shaking diameter 25mL filling volume temperature 37 C Following an initial decline the dissolved oxygen concentration in the medium rises after 7 hours of cultivation indicating that glucose is totally exhausted During this initial growth phase biomass and CO 2 are formed with ethanol as additional by product of the aerobic fermentation It can also be seen that shortly after the yeasts have adapted their metabolism to ethanol consumption the DO decreases until alcohol is completely oxidized In the baffled flask this point was reached after 23 hours of cultivation noticeable as the soaring concentration of dissolved oxygen DO does not drop below 50 saturation during the entire cultivation indicating a sufficient supply of oxygen to the cells Ethanol consumption takes longer in the ordinary flask with the dissolved oxygen concentration dropping below 10 saturation after 16 hours leading to oxygen limitation of the yeast Both carbon sources were only exhausted after 27 hours and the breathability of the yeast cells declined significantly In both types of flask a rise in pH can be observed after complete consumption of the substrates after 23 resp 27 h potentially caused by autolysis of the cells This demonstrates that the BPM system can easily detect online the different stages of fermentation in shake flasks without laborious sampling and subsequent analysis Top Escherichia coli Escherichia coli Overflow metabolism and acetate consumption Because

    Original URL path: http://www.kuhnershaker.us/products/advanced-applications/online-measurement (2016-04-27)
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  • Kuhner Shaker, Inc. - Flask pH and DO Measurement
    v v pH 2 10 Cross sensitivity typically no cross sensitivity in culture media reduced to ionic strength salinity a high concentration of small florescent molecules in the visible range can interfere Storage stability 18 months provided the sensor is stored in the dark 18 months provided the sensor is stored in the dark Calibration sensor flask are pre calibrated one point calibration is possible at the beginning of the experiment when the medium conditions O 2 are defined sensor flask are pre calibrated one point calibration is possible at the beginning of the experiment when the medium conditions pH are defined Sterilization sensor flask are delivered irradiated sensor flask are delivered irradiated Resterilization steam sterilization ethylene oxide gamma irradiation is not recommended Top Application fields Application fields Online measurement of dissolved oxygen and pH S cerevisiae E coli CHO cell culture Saccharomyces cerevisiae Crabtree effect and consumption of ethanol Due to its overflow metabolism Crabtree effect baker s yeast Saccharomyces cerevisiae has the ability to produce ethanol from glucose even in the presence of oxygen After total consumption of the sugar accumulated ethanol can be oxidized as an energy source by baker s yeast under aerobic conditions With the help of the BPM system the impact of baffles on the oxygen supply to yeast cells was investigated The above mentioned diauxic growth of S cerevisiae with glucose and ethanol is illustrated with data generated by the BPM Fig 1 Dissolved oxygen concentration DO and pH value during cultivation of Saccharomyces cerevisiae in shake flasks Corning 250ml with and without baffles YEP medium with 20g L glucose 200rpm shaking frequency 50 mm shaking diameter 25mL filling volume temperature 37 C Following an initial decline the dissolved oxygen concentration in the medium rises after 7 hours of cultivation indicating that glucose is totally exhausted During this initial growth phase biomass and CO 2 are formed with ethanol as additional by product of the aerobic fermentation It can also be seen that shortly after the yeasts have adapted their metabolism to ethanol consumption the DO decreases until alcohol is completely oxidized In the baffled flask this point was reached after 23 hours of cultivation noticeable as the soaring concentration of dissolved oxygen DO does not drop below 50 saturation during the entire cultivation indicating a sufficient supply of oxygen to the cells Ethanol consumption takes longer in the ordinary flask with the dissolved oxygen concentration dropping below 10 saturation after 16 hours leading to oxygen limitation of the yeast Both carbon sources were only exhausted after 27 hours and the breathability of the yeast cells declined significantly In both types of flask a rise in pH can be observed after complete consumption of the substrates after 23 resp 27 h potentially caused by autolysis of the cells This demonstrates that the BPM system can easily detect online the different stages of fermentation in shake flasks without laborious sampling and subsequent analysis Top Escherichia coli Escherichia coli Overflow metabolism and acetate consumption Because

    Original URL path: http://www.kuhnershaker.us/products/advanced-applications/online-measurement/flask-ph-and-do-measurement (2016-04-27)
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  • Kuhner Shaker, Inc. - Respiration Measurement
    shaking diameter 50 mm complex medium with 20 g L glucose After an initial rise the oxygen transfer rate OTR reaches a plateau after 5 hours and remains static on a level of 0 02 mol L 1 h 1 till 21 hours of cultivation This curve is typical for an oxygen limited culture The carbon dioxide transfer rate CTR increases even after 5 hours Due to the limited availability of oxygen the cells must have converted the sugar aerobically as well as anaerobically to CO 2 and ethanol during this time This is demonstrated in figure 1 by the offline determined EtOH concentration After 10 hours primary carbon source glucose is exhausted CTR drops Pichia now begins to degrade the ethanol formed earlier CTR declines below the level of OTR resulting in a respiratory quotient RQ 1 a typical value for carbon sources more reduced than glucose End of fermentation is reached after 21 hours with both OTR and CTR dropping Figure 1 illustrates that online data from RAMOS and offline results complement one another and provide a comprehensive picture of the metabolic state of the culture Gassing in RAMOS measuring flasks can be adapted to the respective type of closure of the parallel sampling flaks so that ventilation in both types of bioreactors is comparable This becomes apparent in terms of the volatile component ethanol Depletion of the offline determined EtOH concentration corresponds with the decline of OTR and CTR in RAMOS flasks Different fermentation stages respiratory fermentative can be exactly detected with RAMOS without need for additional sampling Hence expense of time and money for offline analytics can be diminished RQ can be calculated with OTR and CTR and allows drawing conclusions to the kind of carbon source consumed by the cells Top Corynebacterium glutamicum Recognition of limitations on different levels RAMOS is applicable for media optimization as shown by the following example with Corynebacterium glutamicum a gram positive soil bacterium Fig 2a Oxygen transfer rate of Corynebacterium glutamicum in RAMOS with different filling volumes of the flasks addition of substrate A and or B after 24 h supplement solutions do not contain any carbon source mineral medium with glucose shaking speed 300 rpm shaking diameter 50 mm flask volume 250 mL temperature 30 C in cooperation with IBT2 Forschungszentrum Jülich The flasks in the experiment of figure 2a exhibit different filling volumes from 10 to 20 mL However course of oxygen transfer rates OTR in all flasks is similar OTR mounts at the beginning reaches its maximum after 9 hours to decline in the following This curve is typical for a substrate limited culture Though the carbon source glucose is available in sufficient amounts at that time data not shown Hence growth of C glutamicum must be restricted by the availability of a different nutrient To determine which substrate constitutes the limiting factor at this cultivation two mineral solutions without carbon sources A and B were added solely or in combination Fig 2b Oxygen transfer rate

    Original URL path: http://www.kuhnershaker.us/products/advanced-applications/online-measurement/respiration-measurement (2016-04-27)
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