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  • Kuhner Shaker, Inc. - SFR
    pH and DO Description Characteristics Kuhner shaker Description The Shake Flask Reader SFR is an online measurement that determines valuable parameters such as dissolved oxygen and pH in shake flasks Top Performance characteristics Rapid parallel online monitoring of up to 9 shake flasks For microbial cultivation and cell culture Compatible with Kuhner incubator shakers Non invasive measurement Optimization of growth conditions Top Integration into Kuhner incubator shakers The SFR fits in all Kuhner incubator shakers It is screwed directly on to any Kuhner universal tray EU EXU FU The specialization provides monitoring of oxygen and pH in up to 9 shake flasks simultaneously The corresponding ready to use flasks contain pre calibrated sensor spots The system monitors non invasively through the transparent bottom of the flask Different types and sizes of flasks are available Plastic flasks contain pre calibrated oxygen and pH sensors They are ready to use for one cultivation Glass flasks are equipped with autoclavable oxygen sensors Top Technical Data Technical data Determines online pH and DO Shake flask reader Oxygen sensor pH sensor Shake flask reader SFR Shaker Applicable for all Kuhner shakers V W X series Temperature range 5 50 C Positions 9 Top Oxygen sensor

    Original URL path: http://www.kuhnershaker.us/products/advanced-applications/online-measurement/sfr (2016-04-27)
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  • Kuhner Shaker, Inc. - CTISens Bio
    Top Recording of measured values in real time Real time analysis of measurements relevant to the process takes place in the disposable reactor This eliminates costly and risky sample taking which disturbs the cell system and could lead to contamination With CITSens Bio cell culture sterility is guaranteed at all times The CITSens Bio sensor a screen printed electrode is very easy to handle The gamma sterilised sensor is delivered ready for use The sensor is connected to the CITSens Bio Beamer by a cable The CITSens Bio Beamer is placed right next to the reactor system and incorporates a data acquisition unit as well as a transmitter for wireless transmission of the measured values of glucose glutamate and lactate in g I directly to a receiver ZOMOFI receives measurement data from up to 1 000 sensors and transfers these to the PC RS232 or LAN Sensor with cap Top Technical Data Technical data Online and in situ measurement of glucose glutamate and lactate CITSens Bio Sensor Radio transmitter Radio receiver CITSens Bio Sensor CITSens Bio Sensor Screen printed electrode with immobilized enzyme Precision 1 mmol Resolution glucose 0 1 mmol glutamate 0 5 mmol lactate 0 5 mmol Detection

    Original URL path: http://www.kuhnershaker.us/products/advanced-applications/online-measurement/ctisens-bio (2016-04-27)
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  • Kuhner Shaker, Inc. - Fed-Batch
    FB 12 mm diameter Art Nr SMFB63319 per 250 mL shake flask each with 25 mL 0 1 M phosphate buffer pH 7 temperature 37 C shaking frequency 280 rpm shaking diameter 50 mm Crystalline glucose is embedded in a silicone matrix and released with a defined kinetic The amount of glucose in the experiment can be increased simply by adding multiple beads The beads do not interact Top Hansenula polymorpha Screening under substrate limited conditions The yeast Hansenula polymorpha is a well known protein producing microorganism Green fluorescent protein GFP under control of appropriate promoters is a common reporter protein in Hansenula because it is easy to detect Expression of GFP in Hansenula polymorpha is subject to catabolite repression in the presence of high glucose concentrations The majority of screening experiments are performed in batch mode so GFP expression is limited to the short period before the glucose is almost completely consumed This means it is often impossible to identify the best protein producing clones under substrate limited conditions during a batch screening setup FeedBeads mimic fed batch conditions even under screening conditions Catabolite repression can be circumvented and suitable production clones are rapidly identified Fig 2 Hansenula polymorpha production of green fluorescent protein GFP with slow release substrate FeedBeads and in batch mode control medium SYN6 red with 5 g L glucose without glycerol with 100 mM citrate temperature 30 C shaking speed 800 rpm shaking diameter 3 mm 96 well microtiter plate 6 mm FeedBeads Art Nr SMFB63318 Without FeedBeads the short period between 8 and 12 hours cultivation time in which the yeast is producing GFP is clearly visible Afterwards glucose is consumed completely and the cells metabolism succumbs to the lack of carbon source In contrast the protein production of Hansenula can be significantly enhanced with FeedBeads After the cells have grown actual glucose concentration in the medium is negligible because all the released glucose is expended immediately by the cells FeedBeads supply the yeast cells with a continuous carbon and energy source but the amount of free glucose is too low to induce catabolite repression With the application of FeedBeads catabolite repression in Hansenula polymorpha can be avoided and production of GFP is four times higher when compared with the control All cultures are synchronized and results are reproducible even from microplates Top Escherichia coli FeedBeads prevent aerobic acetate formation If the bacterium Escherichia coli takes up more glucose than the central metabolism can process the excess glucose is converted to acetate even in the presence of oxygen This overflow metabolism occurs at high glucose concentrations After complete consumption of the sugar E coli is able to utilize the acetate formed earlier as a carbon and energy source in a type of diauxic behavior gaining an advantage over competing microorganisms However for various reasons acetate is an unwanted by product in fermentation processes the weak organic acid uncouples the transmembrane pH gradient This interferes with energy metabolism and pH dependent carriers of the cell

    Original URL path: http://www.kuhnershaker.us/products/advanced-applications/fed-batch (2016-04-27)
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  • Kuhner Shaker, Inc. - Feed Beads
    FB 12 mm diameter Art Nr SMFB63319 per 250 mL shake flask each with 25 mL 0 1 M phosphate buffer pH 7 temperature 37 C shaking frequency 280 rpm shaking diameter 50 mm Crystalline glucose is embedded in a silicone matrix and released with a defined kinetic The amount of glucose in the experiment can be increased simply by adding multiple beads The beads do not interact Top Hansenula polymorpha Screening under substrate limited conditions The yeast Hansenula polymorpha is a well known protein producing microorganism Green fluorescent protein GFP under control of appropriate promoters is a common reporter protein in Hansenula because it is easy to detect Expression of GFP in Hansenula polymorpha is subject to catabolite repression in the presence of high glucose concentrations The majority of screening experiments are performed in batch mode so GFP expression is limited to the short period before the glucose is almost completely consumed This means it is often impossible to identify the best protein producing clones under substrate limited conditions during a batch screening setup FeedBeads mimic fed batch conditions even under screening conditions Catabolite repression can be circumvented and suitable production clones are rapidly identified Fig 2 Hansenula polymorpha production of green fluorescent protein GFP with slow release substrate FeedBeads and in batch mode control medium SYN6 red with 5 g L glucose without glycerol with 100 mM citrate temperature 30 C shaking speed 800 rpm shaking diameter 3 mm 96 well microtiter plate 6 mm FeedBeads Art Nr SMFB63318 Without FeedBeads the short period between 8 and 12 hours cultivation time in which the yeast is producing GFP is clearly visible Afterwards glucose is consumed completely and the cells metabolism succumbs to the lack of carbon source In contrast the protein production of Hansenula can be significantly enhanced with FeedBeads After the cells have grown actual glucose concentration in the medium is negligible because all the released glucose is expended immediately by the cells FeedBeads supply the yeast cells with a continuous carbon and energy source but the amount of free glucose is too low to induce catabolite repression With the application of FeedBeads catabolite repression in Hansenula polymorpha can be avoided and production of GFP is four times higher when compared with the control All cultures are synchronized and results are reproducible even from microplates Top Escherichia coli FeedBeads prevent aerobic acetate formation If the bacterium Escherichia coli takes up more glucose than the central metabolism can process the excess glucose is converted to acetate even in the presence of oxygen This overflow metabolism occurs at high glucose concentrations After complete consumption of the sugar E coli is able to utilize the acetate formed earlier as a carbon and energy source in a type of diauxic behavior gaining an advantage over competing microorganisms However for various reasons acetate is an unwanted by product in fermentation processes the weak organic acid uncouples the transmembrane pH gradient This interferes with energy metabolism and pH dependent carriers of the cell

    Original URL path: http://www.kuhnershaker.us/products/advanced-applications/fed-batch/feed-beads (2016-04-27)
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  • Kuhner Shaker, Inc. - Gas mixing
    equipment family incubator shakers disposable bioreactors Top Performance characteristics Mixing up to four gases also gas mixtures can be connected Selectable flow rate 0 1 sL min 0 20 sL min 0 200 sL min ISIS Software Integration in Kuhner shakers Integrated pressure sensor Top Compatible with different bioreactors a Stirred tank bioreactor b Other disposable systems c Orbital shaken bioreactor Top Reproducibility Up to four mass flow controllers ensure high accuracy and reproducibility of the gas mixture The number of mass flow controllers can be adapted to individual requirements FlowCon 2 3 4 An integrated pressure sensor prevents overpressure in the connected bioreactor by reducing the gas flow in the first step and shutting off the gas flow in the second step Step limits can be freely programmed by the user Integrated air filters and back pressure valves ensure a long interference free operating time of the FlowCon The Kuhner ISIS software provides data logging programming and controlling Technical controls for 21 CFR Part 11 compliance are built in to ISIS X software Top Technical Data Technical data Stand alone gas mixing device FlowCon 2 3 4 FlowCon 2 3 4 Number of mass flow controllers 2 3 or

    Original URL path: http://www.kuhnershaker.us/products/advanced-applications/gas-mixing (2016-04-27)
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  • Kuhner Shaker, Inc. - FlowCon 2/3/4
    equipment family incubator shakers disposable bioreactors Top Performance characteristics Mixing up to four gases also gas mixtures can be connected Selectable flow rate 0 1 sL min 0 20 sL min 0 200 sL min ISIS Software Integration in Kuhner shakers Integrated pressure sensor Top Compatible with different bioreactors a Stirred tank bioreactor b Other disposable systems c Orbital shaken bioreactor Top Reproducibility Up to four mass flow controllers ensure high accuracy and reproducibility of the gas mixture The number of mass flow controllers can be adapted to individual requirements FlowCon 2 3 4 An integrated pressure sensor prevents overpressure in the connected bioreactor by reducing the gas flow in the first step and shutting off the gas flow in the second step Step limits can be freely programmed by the user Integrated air filters and back pressure valves ensure a long interference free operating time of the FlowCon The Kuhner ISIS software provides data logging programming and controlling Technical controls for 21 CFR Part 11 compliance are built in to ISIS X software Top Technical Data Technical data Stand alone gas mixing device FlowCon 2 3 4 FlowCon 2 3 4 Number of mass flow controllers 2 3 or

    Original URL path: http://www.kuhnershaker.us/products/advanced-applications/gas-mixing/flowcon-2-3-4 (2016-04-27)
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  • Kuhner Shaker, Inc. - Duetz System
    roundwell MTPs from Corning SMCR1524a Sandwich cover for polystyrene 24 low roundwell MTPs from Corning low evaporation version of SMCR1524 Top For 48 deepwell MTPs SMCR1248 Sandwich cover for 48 deepwell MTPs from DOT Scientific with spongy silicone 1 2 mm holes Top For 96 deepwell MTPs SMCR1296 Sandwich cover for all types of 96 deepwell MTPs with spongy silicone 1 5 mm holes SMCR1296a Sandwich cover for all types of 96 deepwell MTPs with solid silicone 0 8 mm holes low evaporation SMCR1296b Sandwich cover for Greiner 96 deepwell MTPs and SMCR1496a with spongy silicone fixed with pins 1 5 mm holes SMCR1296c Sandwich cover for Greiner 96 deepwell MTPs and SMCR1496a with solid silicone fixed with pins 0 8 mm holes low evap SMCR1306 Sandwich cover for polystyrene 6 lowwell MTPs with spongy silicone 1 2 mm holes SMCR1396 Universal sandwich cover for 96 well MTPs with solid silicone 0 8 mm holes SMCR1396a Universal sandwich cover for 96 well MTPs with spongy silicone 1 5 mm holes SMCR1396b Sandwich cover for SMCR1496a SMCR1496c and Greiner MTPs with solid silicone fixed with pins 0 8 mm holes low evap SMCR1396c Sandwich cover for SMCR1496a SMCR1496c and Greiner MTPs with spongy silicone fixed with pins 1 5 mm holes SMCR1596 Sandwich cover for polystyrene 96 low roundwell MTPs only suitable for MTPs with empty spaces between wells Top SMCR1306 Sandwich cover for polystyrene 6 lowwell MTPs with spongy silicone 1 2 mm holes Top Replacement parts SMCR0001 20x blue microfiber filter inlay for all types of deepwell covers and universal covers SMCR0002 20x green ePTFE filter inlay for all types of deepwell covers and universal covers SMCR0003 20x blue microfiber filter inlay for SMCR1524 and SMCR1524a covers for Corning MTPs SMCR1524 SMCR0004 20x green ePTFE filter inlay for SMCR1524 and SMCR1524a covers for Corning MTPs SMCR1524 SMCR0005 20x blue microfiber filter inlay for SMCR1596 SMCR0006 20x green ePTFE filter inlay for SMCR1596 SMCR0007 5x Replacement spongy silicone seal 2 5 mm holes for sandwich cover for 24 deepwell MTP SMCR1224 SMCR1224b SMCR0007a 5x Replacement spongy silicone seal 1 2 mm holes for sandwich cover 24 well MTP SMCR1224a SMCR1224c SMCR1324d SMCR0008 5x Replacement solid silicone seal 2 5 mm holes for universal cover 24 well MTPs 19 5 mm hole distance SMCR1324 SMCR0008a 5x Replacement solid silicone seal 1 2 mm holes for universal cover 24 well MTPs 19 5 mm hole distance SMCR1324a SMCR0008b 5x Replacement solid silicone seal 2 5 mm holes for universal cover 24 well MTPs 18 mm hole distance SMCR1324b SMCR0008c 5x Replacement solid silicone seal 1 2 mm holes for universal cover 24 well MTPs 18 mm hole distance SMCR1324c SMCR0008d 5x Replacement spongy silicone seal 1 2 mm holes for universal cover 24 well MTPs 19 5 mm hole distance SMCR1324e SMCR0009 5x Replacement spongy silicone seal for sandwich cover for 24 lowwell MTP SMCR1524 SMCR0009a 5x Replacement solid silicone seal for sandwich cover for 24 lowwell MTP low evap SMCR1524a SMCR0010

    Original URL path: http://www.kuhnershaker.us/products/advanced-applications/duetz-system (2016-04-27)
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  • Kuhner Shaker, Inc. - Duetz-System
    roundwell MTPs from Corning SMCR1524a Sandwich cover for polystyrene 24 low roundwell MTPs from Corning low evaporation version of SMCR1524 Top For 48 deepwell MTPs SMCR1248 Sandwich cover for 48 deepwell MTPs from DOT Scientific with spongy silicone 1 2 mm holes Top For 96 deepwell MTPs SMCR1296 Sandwich cover for all types of 96 deepwell MTPs with spongy silicone 1 5 mm holes SMCR1296a Sandwich cover for all types of 96 deepwell MTPs with solid silicone 0 8 mm holes low evaporation SMCR1296b Sandwich cover for Greiner 96 deepwell MTPs and SMCR1496a with spongy silicone fixed with pins 1 5 mm holes SMCR1296c Sandwich cover for Greiner 96 deepwell MTPs and SMCR1496a with solid silicone fixed with pins 0 8 mm holes low evap SMCR1306 Sandwich cover for polystyrene 6 lowwell MTPs with spongy silicone 1 2 mm holes SMCR1396 Universal sandwich cover for 96 well MTPs with solid silicone 0 8 mm holes SMCR1396a Universal sandwich cover for 96 well MTPs with spongy silicone 1 5 mm holes SMCR1396b Sandwich cover for SMCR1496a SMCR1496c and Greiner MTPs with solid silicone fixed with pins 0 8 mm holes low evap SMCR1396c Sandwich cover for SMCR1496a SMCR1496c and Greiner MTPs with spongy silicone fixed with pins 1 5 mm holes SMCR1596 Sandwich cover for polystyrene 96 low roundwell MTPs only suitable for MTPs with empty spaces between wells Top SMCR1306 Sandwich cover for polystyrene 6 lowwell MTPs with spongy silicone 1 2 mm holes Top Replacement parts SMCR0001 20x blue microfiber filter inlay for all types of deepwell covers and universal covers SMCR0002 20x green ePTFE filter inlay for all types of deepwell covers and universal covers SMCR0003 20x blue microfiber filter inlay for SMCR1524 and SMCR1524a covers for Corning MTPs SMCR1524 SMCR0004 20x green ePTFE filter inlay for SMCR1524 and SMCR1524a covers for Corning MTPs SMCR1524 SMCR0005 20x blue microfiber filter inlay for SMCR1596 SMCR0006 20x green ePTFE filter inlay for SMCR1596 SMCR0007 5x Replacement spongy silicone seal 2 5 mm holes for sandwich cover for 24 deepwell MTP SMCR1224 SMCR1224b SMCR0007a 5x Replacement spongy silicone seal 1 2 mm holes for sandwich cover 24 well MTP SMCR1224a SMCR1224c SMCR1324d SMCR0008 5x Replacement solid silicone seal 2 5 mm holes for universal cover 24 well MTPs 19 5 mm hole distance SMCR1324 SMCR0008a 5x Replacement solid silicone seal 1 2 mm holes for universal cover 24 well MTPs 19 5 mm hole distance SMCR1324a SMCR0008b 5x Replacement solid silicone seal 2 5 mm holes for universal cover 24 well MTPs 18 mm hole distance SMCR1324b SMCR0008c 5x Replacement solid silicone seal 1 2 mm holes for universal cover 24 well MTPs 18 mm hole distance SMCR1324c SMCR0008d 5x Replacement spongy silicone seal 1 2 mm holes for universal cover 24 well MTPs 19 5 mm hole distance SMCR1324e SMCR0009 5x Replacement spongy silicone seal for sandwich cover for 24 lowwell MTP SMCR1524 SMCR0009a 5x Replacement solid silicone seal for sandwich cover for 24 lowwell MTP low evap SMCR1524a SMCR0010

    Original URL path: http://www.kuhnershaker.us/products/advanced-applications/duetz-system/duetz-system (2016-04-27)
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